COLOR CODE FOR JSG

Sept/Oct 2006

DHH using Sulston’s notebook & Lints’ notebook

This L4 male tail was lineaged prior to fixation for TEM, allowing Sulston to compare cell fates to known lineage positions within the developing tissues. Many of the cell positions shown in Sulston et al. (1980) are derived from this animal (Fig’s 7, 8, 9, 12, 16, 19, 20, 28). At the time of fixation, cell divisions seem to be complete, and morphogenesis of male features is still underway, with features of ray formation caught “in the act”. Note the developing fan cuticle is still confined within the L4 body cuticle at this stage. All color codes date from the work by Sulston; a few labels were added by R. Lints to mark ray cell groups.

Lineages

RAYS and lumbar neurons

[n.b. there was an overlapping use of red numbers again for JSG’s ventral cord]

1 PHBR

2 PHAR

3 Phso1R

4 Rn8BR

5 Rn8AR

6 Rn9BR

7 Rn9AR

8 Rn9stR

9 Rn8stR

10 Left side neuron

11 PHBL

12 PHAL

13 PHso1L

14 PHshL

15 PHso2L

16 PVQL?

17 PHshR

18 PHso2R

19 Rn9BL

20 Rn9AL

21 Rn9stL

22 Rn8BL

23 Rn8AL

24 Rn8stL

25 Rn7BL

26 Rn7AL

27 Rn7stL

28 Rn7BR

29 Rn7AR

30 Rn7stR

31 Rn5BR

32 Rn5AR

33 Rn5stR

34 Rn6BL

35 Rn6AL

36 Rn6stL

37 Rn4BR

38 Rn4AR

39 Rn4stR

40 Rn6BR

41 Rn6AR

42 Rn6stR

43 neuron process?

44 PVR?

45 Rn3BR

46 Rn3AR

47 Rn3stR

48 neuron in right lumbar ganglion

49 neuron in right lumbar ganglion

50 neuron in right lumbar ganglion

51 neuron in right lumbar ganglion

52 neuron in right lumbar ganglion

53 neuron in right lumbar ganglion

54 neuron in right lumbar ganglion

55 neuron in right lumbar ganglion

56 neuron in right lumbar ganglion

57 Rn5AL

58 Rn5BL

59 Rn5stL

60 Rn4BL

61 Rn4AL

62 Rn4stL

63 Rn3stL

64 neuron in left lumbar ganglion

65 neuron in left lumbar ganglion

66 neuron in left lumbar ganglion

67 neuron in left lumbar ganglion

68 neuron in left lumbar ganglion

69 neuron in left lumbar ganglion

70 neuron in left lumbar ganglion

71 neuron in left lumbar ganglion

72

73 Rn3BL

74 Rn3AL

1 neuron process running from dorsal cord into DRG

2

3 neuron process running from dorsal cord into DRG

4 dorsal cord process

5 dorsal cord process

6 PCAL C.plppd

7 PVX P12.aap

8 HOA P10.pppa

9 HOB P10.ppap

10 SPCL B.alpaap

11 SPVL Bb.lda

12 PCsoL socket C.plap

13 PCCR B.alpaaa

14 PCBL C.plpa

15 PCshL sheath C.plppv

16 PCh hypodermis C.plaa

17 SPso1L Bg.pl

18 SPDL B.alpapaa

19 neuron process running in DRG

20 DX1/2 F.lvda

21 SPso1R Bg.pr

22 DX1/2 F.rvda

23 EF1/2 F.rvdp

24 EF1/2 F.lvdp

25 K.p K.p

26 neuron in DRG

27 DVE B.ppap

28 neuron in DRG

X

29 DVF B.ppppa

30 ventral hyp P10.papp

31 PVV P12.aap

32 PDC? P11.papa

33 hypodermal; then becomes PDA C.a

34 PVV P11.paaa

35 PVY P11.paap

36 PVS?

37 VD13 P12.app

38 DA9?

39 PGA? daughter of P11

40 SPshVL Bb.ldp

41 SPso4L B.alpapp

42 SPshDL B.alpapap

43 SPso3L Bb.lv

44 SPso2L Ba.ld

45 hyp associated with left spicule

46 hyp associated with left spicule

50 SPCR B.arpaap

51 SPVR Bb.rda

52 SPDR B.arpapaa

53 PCsoR socket C.prap

54 PCCL B.arpaaa

55 PCAR C.prppd

56 PCBR C.prpa

57 PCshR sheath C.prppv

58 PCh hypodermis C.praa

59 SPso4R B.arpapp

60 SPshDR B.arpapap

61 SPshVR Bb.rdp

62 SPso3R socket Bb.rv

63 SPso2R socket Ba.rd

64 hyp associated with right spicule

65 hyp associated with right spicule

66 right spicule (hyp?)

67 DX3 E.raa

68 EF3 E.rap

69 DA8

70 HOso socket P10.ppaa

71 hook epithelium P10.papp

72 preanal hyp P10 daughter

73 HOsh sheath P10.ppppp

74

75 VA12 P12.aaaa

76 neuron in PAG

77 DD6

78 PVT

79 VD12 P11.app

80 AS11 P11.apa

81 VA11 P11.aaaa

82 CA9 or CP9?? P11 daughter

1 proctodeum (epithelium) B.pap

2 proctodeum (epithelium) B.paa

3 proctodeum (epithelium) Bd.r

4 proctodeum (epithelium) Bd.l

5 proctodeum (epithelium) B.arapaad

6 proctodeum (epithelium) B.alapaad

7 proctodeum (epithelium) F.rvv

8 proctodeum (epithelium) F.lvv

9 proctodeum (epithelium) Bg.ald

10 proctodeum (epithelium) Bg.alv

11 proctodeum (epithelium) Ba.arv

12 proctodeum (epithelium) Ba.alv

13 proctodeum (epithelium) Bg.arv

14 fused engulfing cell F.ld

15 fused engulfing cell F.rd

16 cells that will die Bg.ard or Bg.ald

17 proctodeum (epithelium) B.pppa

18 proctodeum (epithelium) B.ppppp

19 vas epithelium cells that will die B.arapaav and B.alapaav

20 K.a K.a

21 gonad leader (linker cell) [is engulfed]

22 killer of linker cell E.rp

23 engulfer cell (fused) E.ra

24 engulfer cell (fused) E.lp

25 tail ventral hyp [2 nuclei]

26 tail hyp anal ridge [4 nuclei] hyp10, hyp9, hyp8

27 hyp12 P12.pa

28 hook sensillum associated P11.ppap

29 hook sensillum associated P11.ppp

30 K’ K’

31 recD dorsal rectal gland

32 recL left rectal gland

33 recR right rectal gland

34 virL rectal valve

35 virR rectal valve

36 left set SET

37 right set SET

38 right Y.p R8.p

39 left Y.p R8.p

40 right Z.p R9.p

41 left Z.p R9.p

42 hyp 11

43 right X.p R7.p

44 left X.p R7.p

45 right W R5.p

46 left W R5.p

47 right U.p R4.p

48 left U.p R4.p

49 hyp7 right side T.aa

50 hyp7 left side T.aa

51 right S Rn.p?

52 left S Rn.p?

53 Rn.p or hyp7?

Sex Muscles & Bodywall Muscles (N.B. same code as for N2Y)

1 dorsal bodywall muscle

2 caudal longitudinal muscle L

3 caudal longitudinal muscle R

4 ventral bodywall muscle?

5 ventral bodywall muscle?

6 dorsal bodywall muscle

7 gubernacular erector L

8 dorsal bodywall muscle

9 gubernacular erector R

10 anal depressor muscle

11 dorsal spicule protractor L

12 dorsal spicule protractor R

13 ventral spicule protractor L

14 ventral spicule protractor R

15 dorsal spicule retractor L

16 dorsal spicule retractor R

17 ventral spicule retractor L

18 ventral spicule retractor R

19 posterior inner longitudinal L

20 anterior inner longitudinal R

21 posterior inner longitudinal R

22 anterior inner longitudinal L

23 U

27 anterior outer longitudinal R

30 dorsal bodywall muscle

31 dorsal bodywall muscle

32 dorsal bodywall muscle

33 dorsal bodywall muscle

34 dorsal bodywall muscle

35 dorsal bodywall muscle

36 dorsal bodywall muscle

37 dorsal bodywall muscle

38 dorsal bodywall muscle

39 sphincter muscle generally not labelled

44 anterior outer longitudinal L

47 posterior outer longitudinal L

49 anterior oblique R

50 gubernacular retractor L

51 posterior oblique R

52 posterior oblique L

53 gubernacular retractor R

54 anterior oblique L

55 diagonal muscle R

56 diagonal muscle L

57 diagonal muscle R

58 diagonal muscle L

59 diagonal muscle R

60 diagonal muscle L

61 diagonal muscle R

62 diagonal muscle L

63 diagonal muscle R

64 diagonal muscle L

65 diagonal muscle R

66 diagonal muscle L

67 diagonal muscle R

68 diagonal muscle L

69 diagonal muscle R

74 posterior outer longitudinal R